Part:BBa_K257010:Design

clyA + RFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 84
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1494
  Illegal AgeI site found at 1606
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

To avoid frameshift between clyA fusion C Term and RFP fusion N Term we use respectively the fusion silver standard suffix 23 (for clyA C term, the prefix being standard 10) and the fusion silver standard prefix 23 (for RFP N Term, the suffix being standard 10)

Source

Assembly of BBa_K257002 and BBa_K257008.